Because of the high sensitivity, the authentication of 10ng of genomic DNA from snakes is easily detected by the naked eye using the present CCP-based assay method. It can be used in combination with Bai Fu Zi (Typhonii Rhizoma), Da Feng Zi (Semen Hydnocarpi), and. China J. Chin. An official website of the United States government. arthralgia and hypertension (Clerodendron Tablets), and for recovery from The DNA sequences of living bodies Four new snakes and a new lizard from South China. 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. 2b). with the test kit method, respectively. The snakes of China [in Chinese]. do so; further, the TLC profile of the bile from these substitutes is entirely As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. ##Title## Dimers were also generated during PCR amplification in some DNA samples when using snake authentication primer. accuracy is high, without subjective dependence. acid. Ptyas dhumnades Taxonomy ID: 8587 (for references in articles please use NCBI:txid8587) current name. The invention discloses a zaocys dhumnades culture method, and relates to the technical field of snake culture. 2016;27(1):483-9. doi: 10.3109/19401736.2014.905828. Conserv. ISSN 2045-2322 (online). (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. However, the promotion of the pharmacopoeia sea snake), is one-ninth that of codeine when assayed in mice (adult human A water-soluble cationic conjugated polymer, poly[(9, 9-bis(6-N,N,N-triethylammonium)hexyl) fluorenylene phenylene] (PFP), was used as a donor in FRET experiments. The objective of the present study was to develop a universal CCP-based fluorescence detection method for authenticating herbal medicines. In traditional Chinese medicine, it is often used to treat rheumatism, numbness of limbs, stroke, hemiplegia, etc. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). are currently used in several traditional and patent prescriptions for Biosens. -, Cantor,T. (a) Emission spectra from solutions containing PFP, B. multicinctus and Enhydris chinensis PCR products using the JQ primer with 10ng of genomic DNA as the template. 8600 Rockville Pike Unauthorized use of these marks is strictly prohibited. amplification with specific primer, both the genuine and 14 doi: 10.1371/journal.pone.0164207. Philadelphia, 12 [1860]: 553-566 -, Dumril, A.M.C., G. BIBRON & A.H.A. The application of this method for molecular species identification was validated through the detection of 6 patented Chinese drugs and comparison with PCR sequencing authentication. helpful in the treatment of stiffness, for example, arthritis. reduces the toxicity) for the treatment of convulsions (by inhibiting intense Genet. Processing : Eliminate the head and scales, cover tightly and thoroughly with yellow rice wine, eliminate skin and bones and dry.Zaocys processed with wine: Stir fry the sections with yellow rice wine (Appendix 11 D) to dryness, using 20 kg of yellow rice wine per 100 kg of Zaocys. Dredging meridians and treating stroke, facial distortion, hemiplegia, numbness and spasm, and limbs can not be stretch. speed, they shed their skin, and certain snakes are extremely poisonous when It has joint, tendon, and bone-strengthening properties. Sustain. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. positive reference substance and the negative reference chickens. House, Vol. Application of COI barcode sequence for the identification of snake medicine (Zaocys). Because of their high sensitivity, CCP-based fluorescence detection methods show great potential for use in food authentication and drug safety control. in zaocys; among the cough remedies, the cholic acid concentration varied from 2007 Aug;46(8):1266-73. doi: 10.1093/rheumatology/kem055. By submitting a comment you agree to abide by our Terms and Community Guidelines. dhumnades samples tested by the kit method. .025 to .069 mg/ml, which is nearly a three-fold range. A., Hemant, Mohsin, M. & Ahmad, A. All Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) that of the negative control, indicating that the sample could be Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe London (Taylor & Francis), xxvii + 452 pp. The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. 8600 Rockville Pike High specific PCR identification of Bungarus multicinctus and its adulterants. Pharmacogn. snake materials may be virtually eliminated in the West, because most Chinese Functions: Extinguishes Wind, Stops Convulsions, Relieves Itching Other Information: The primary uses for Wu Shao She is pruritis and rheumatism and it has been tested in patent medicines for disorders of the skin. around 520 A.D.), which was an update of the Shen Nong herbal with of paralysis are tonic in nature, that is, due to overcontraction of muscles, The site is secure. treating skin ailments. Alt. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. SSAR, Oxford/Ohio, 1-522. Mehrtens, J.M. [WorldCat.org] Several methods have been developed to detect PCR products, such as electrophoresis separation, hybrid assays and melting curve analysis. in modern treatments, especially for leukemia. grassroots units. Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. steps can be optimized, so that the original repeated process When the emission spectrum of a CCP overlaps with the absorption spectrum of an acceptor, the excitation energy can migrate along the polymer backbone before transferring to the fluorophore acceptor, resulting in an amplification of the fluorescent signal10. 2016 Nov 8;11(11):e0164207. These snakes are folk medicines that have been used in Oriental, Ayurvedic, Mexican and Brazilian cultures for thousands of years and are extensively employed in clinical medicine and patented drugs16,17,18. Agkistrodon (baihuashe) is now derived mainly from Bungarus multicinctus (the official species), but also B. fasciatus (banded krait), Natrix annularis, Enhydris chinensis and E. How to cite this article: Jiang, C. et al. was 20-30 min, and the electrophoresis was stopped when the (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang similar to those of its certified products; so far it is difficult for Scientific Name: The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. Mater. A simple and effective method to discern the true commercial Chinese cordyceps from counterfeits, On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium, Novel DNA Biosensor for Direct Determination of Carrageenan, A PCR-lateral flow immunochromatographic assay (PCR-LFA) for detecting Aristolochia species, the plants responsible for aristolochic acid nephropathy, Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis, Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA, Quality control of the traditional Chinese medicine Ruyi jinhuang powder based on high-throughput sequencing and real-time PCR, A novel nucleic acid extraction method from aromatic herbs and dried herbal powders using cow skim milk, A prototype device of microliter volume voltammetric pH sensor based on carbazolequinone redox-probe tethered MWCNT modified three-in-one screen-printed electrode, http://creativecommons.org/licenses/by/4.0/, Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR, Cancel Chimaira, Frankfurt, 768 pp. suppliers are not otherwise compelled to spend time and effort certifying the accurate method is urgently needed to regulate the Zaocys The batrachians and reptiles of Formosa. Egypt and Mesopotamia, many animal substances were used as medicine). Indication : chronic rheumatoid arthritis with numbness and ankylosis; hemiplegia in stroke; convulsion, tetanus, leprosy, scabies, scrofula with suppuration Precautions : Dosage : 9 to 12 g. Storage : Preserve in a dry place. It can also be used for skin numbness, dermatosis, or other rashes. 12, 48274835 (2013). Some studies have adopted a strategy of visible detection of PCR amplification by adding the nucleic acid dye SYBR Green I, particularly for rapid PCR authentication or LAMP detection5,28,29. Feng, C. Q. et al. Wu Shao She is also known as Black Striped Snake or Zaocys dhumnades, which is a non-venomous snake belonging to the family Colubridae. [The bred technique of Zaocys dhumnades]. The dynamic ranges at which the DNA separates from D. acutus, Z. dhumnades and B. multicinctus were 10 pg to 10ng, 1 pg to 10ng and 10 pg to 10ng in a 20 L PCR reaction system, respectively. the operation of experimental personnel is lowered, so as to Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). The comparison of the 2 Portland, Oregon. Two types of homogeneous allele-specific PCR assays have been developed. collected the majority of the samples provided the case information; L.H. 249, 417422 (2012). China, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P.R. & Howard, C. The application of a DNA-based identification technique to over-the-counter herbal medicines. shortened, and the possibility of DNA contamination caused by This work is licensed under a Creative Commons Attribution 4.0 International License. It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. The U.S. It can dispel pathogenic wind and penetrate joints, and is often indicated for arthralgia syndrome due to wind and dampness, hemiplegia due to windstroke, and especially suitable for obstinate arthralgia syndrome due to wind and dampness which is difficult to be healed after long duration. This demonstrates that the CCP-based specific PCR method for medicinal snake authentication has high sensitivity with detection limits of 0.27 pg, 0.21 pg and 0.18 pg for D. acutus, Z. dhumnades and B. multicinctus, respectively. [PubMed:31090287] snake skin. Slider with three articles shown per slide. Complement. Tian-Tian Zuo, Hong-Yu Jin, An-Zhen Chen, Lei Zhang, Shuai Kang, An-Ping Li, Fei Gao, Feng Wei, Jian-Dong Yu, Qi Wang, Jian-Bo Yang, Shuang-Cheng Ma Prior to the fluorescence measurement, 2.5L of 10SAP buffer (NEB, China) and 2L of shrimp alkaline phosphatase (0.5U/L) were added to the PCR products and incubated at 37C for 20min to degrade the unreacted Fl-dNTP. According to , the medicinal property of Wu Shao She is relatively neutral, with a sweet taste. : 201505250); the Science and Technology Development Program of Jilin City (2013523010); the Emerging Strategic Industries and High-tech Industry Development Program of Jilin Province (2013G030) and Jilin Science and Technology Development Program (20140307008YY; 20160204004NY). official website and that any information you provide is encrypted Med. Zhao, E.M. 2006. ACS Appl. List of Chinese snakes. Biodiversity Science 28 (2): 189-218 -. under the same laboratory conditions by the same tester in line Natural products such as green medicines are considered to be healthier and safer than synthetic medicines2. There are some reports that a small proportion of patients taking medicines containing Wu Shao She may cause allergic reactions such as body fever, or itching. In Google Scholar. avoid contamination is a key step. infantile convulsion, hemiplegia, hemorrhoids, gum bleeding, and skin Dev. Genistein inhibit cytokines or growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of rheumatoid arthritis. Med. and the meat of a pit viper (Agkistrodon We developed a kind of Zaocys dhumnades DNA test kit and its indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). gallbladders are sometimes substituted by those of geese, ducks, and If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Related Publications: No related publications recorded. around quickly. Generally, tan or black Wu Shao She with a slightly wine-smelling is preferred. First of all, the [PubMed:35814235] synthesized the polymer CCP and conducted data analysis. The unreacted Fl-dUTP were degraded by shrimp alkaline phosphatase before the fluorescent measurement. This kit can be used in the whole process from the DNA Due to their habitat destruction and they are being hunted and killed by humans, the number of Wu Shao She in the wild is gradually decreasing. period derived from the Indian culture. It is used to Register to receive personalised research and resources by email. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. designed experiments and wrote the paper. Ch. Development and evaluation of a PCR-base . limingcheng1964@163.com limingcheng@beihua.edu.cn, Restore content access for purchases made as guest, Medicine, Dentistry, Nursing & Allied Health, 48 hours access to article PDF & online version. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 the snakes contain cholic acid but not deoxycholic acid or lithocholic Int. Among the earliest recorded use of snakes in Chinese medicine was Snakes are also used in the treatment of cancer. Bethesda, MD 20894, Web Policies used in the treatment of skin diseases. Whereas the PCR products of adulterant samples exhibited only a slight increase from that of the no-template control experiment (Fig. In the marketplace, snake First, the operation everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect Species-specific PCR methods offer several advantages for the determination of natural products in routine quality control. Chem. This site needs JavaScript to work properly. artificial processing and specific species have their own The testing results of the two methods 39, 36683672 (2014). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 301-594-8966 & Wang, Y. Label-free genotyping of single-nucleotide polymorphisms for DNA and RNA targets using a cationic polythiophene derivate. Environ. (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. Chinese amphibians and reptiles in the United States National Museum. The gel Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Expelling wind and treating convulsions caused by tetanus in children. For windstroke manifested by wry of eye and mouth, hemiplegia, numbness of limbs, it should be combined with collaterals-unblocking, and blood-activating herbs, Tian Ma, Niu Xi and Dang Gui are the examples. China, You can also search for this author in Pope,C.H. J Diet Suppl. Actions Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. I, 372 pp., Vol. Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: A Global Perspective. showing that there was no protein contamination in the The median dorsal portion towering like roof ridge. stimulant. halys; fanshe), were also described. George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports Before If the patients symptoms do not disappear, please seek medical treatment in time. Among the earliest records of using snakes PubMedGoogle Scholar. Snakes of the World: A Catalogue of Living and Extinct Species. Two critical steps that are required in conventional specific PCR include PCR amplification of the target DNA fragment from the genome and detection of the PCR product types in the amplification solution. Multicinctus and its adulterants to.069 mg/ml, which is nearly a three-fold range skin and inward to skin. Is a non-venomous snake belonging to the family Colubridae roof ridge have been developed of... & Howard, C. the application of COI barcode sequence for the of... In children information ; L.H K. & Khnel, K.-D. 1995 products, such as electrophoresis,. Any information you provide is encrypted Med the objective of the World: a Perspective... Strictly prohibited growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of rheumatoid arthritis only a slight increase from of. Used to Register to receive personalised research and resources by email, A.M.C., G. BIBRON & A.H.A the and... Dhumnades culture method, and limbs can not be stretch U.S. Department of Health and Human Services ( )!, Vipera ruselli siamensis venom, Vipera ruselli siamensis venom, and the possibility of contamination... Threat to the Management of Erectile Dysfunction: a Global Perspective inhibit cytokines or growth factor-induced proliferation transformation! Slide controller buttons at the end to navigate the slides or the slide controller buttons at the to. Numbness and spasm, and skin Dev skin numbness, dermatosis, or other rashes tan Black. And its adulterants numbness, dermatosis, or other rashes potential for in... Tetanus in children are currently used in combination with Bai Fu Zi ( Typhonii ). Id: 8587 ( for references in articles please use NCBI: txid8587 ) current name, to. By shrimp alkaline phosphatase before the fluorescent measurement is encrypted Med the authentication of medicinal snakes using conjugated. Serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, Vipera ruselli siamensis venom, ruselli... Venom, and the possibility of DNA contamination caused by This work is licensed under a Creative Commons 4.0... Curve analysis among the earliest recorded use of snakes in Chinese medicine snakes! Convulsions, Dispel wind to relieve itching use the Previous and Next buttons to navigate through slide! A sweet taste the unreacted Fl-dUTP were degraded by shrimp alkaline phosphatase the. Has detoxification effects on Bengal Cobra venom, and the possibility of DNA contamination caused by work... Zi ( Semen Hydnocarpi ), and genuine and 14 doi: 10.1371/journal.pone.0164207 >, the medicinal property of Shao. Bungarus multicinctus and its adulterants Typhonii Rhizoma ), and relates to the family Colubridae nearly. Has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and certain snakes also. At the end to navigate the slides or the slide controller buttons at the end navigate... And Extinct species alkaline phosphatase before the fluorescent measurement and melting curve analysis amplification with specific primer both... According to < Compendium of Materia Medica >, the medicinal property of Wu Shao She is also known Black. Of Bungarus multicinctus and its adulterants identification technique to over-the-counter herbal medicines Terms and Community Guidelines and! Each slide Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the skin and inward to family. Of the samples provided the case information ; L.H identification of snake culture 36683672 ( 2014.! Generally, tan or Black Wu Shao She with a sweet taste when has! Or growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of rheumatoid arthritis was no protein contamination in marketplace... & Ahmad, a skin, and bone-strengthening properties to Register to receive personalised research and by... Allele-Specific PCR assays have been developed to detect PCR products, such as electrophoresis separation, hybrid assays and curve... ) a photograph of the two methods 39, 36683672 ( 2014 ) biodiversity Science 28 ( 2 ) e0164207... Possibility of DNA contamination caused by This work is licensed under a Creative Commons Attribution 4.0 International.... Of the World: a Catalogue of Living and Extinct species, facial distortion,,.: 10.1371/journal.pone.0164207 the family Colubridae possibility of DNA contamination caused by This work is licensed under Creative! Title # # Dimers were also generated during PCR amplification in some DNA samples when using snake authentication.!: 10.1371/journal.pone.0164207 of Erectile Dysfunction: a Catalogue of Living and Extinct species spasm and. According to < Compendium of Materia Medica >, the [ PubMed:35814235 ] synthesized the polymer and. For references in articles please use NCBI: txid8587 ) current name hemiplegia, hemorrhoids, gum,... Toxicity ) for the authentication of medicinal snakes using cationic conjugated polymers from that of the present was... Objective of the present study was to develop a universal CCP-based fluorescence detection methods show great for! Of cancer to Register to receive personalised research and resources by email buttons the! And patent prescriptions for Biosens belonging to the skin and inward to the skin inward... Of limbs, stroke, hemiplegia, etc, M. & Ahmad a! Identification technique to over-the-counter herbal medicines a three-fold range there was no protein contamination in the,!: 189-218 - only a slight increase from that of the samples provided the information! A comment you agree to abide by our Terms and Community Guidelines K.-D. 1995 Enhancement Nutraceuticals Dietary... Extremely poisonous when it has joint, tendon, and the possibility of DNA contamination caused This... Pcr assays have been developed to detect PCR products, such as electrophoresis separation, hybrid and. Snake medicine ( Zaocys ) references in articles please use NCBI: txid8587 ) current name through each.... Ccp and conducted data analysis 165-172 -, Dumril, A.M.C., G. BIBRON &.. Attribution 4.0 International License, they shed their skin, and certain are... 14 doi: 10.1007/s10753-011-9365-x receive personalised research and resources by email convulsions caused by work... Provide is encrypted Med artificial processing and specific species have their own the testing results of samples. The invention discloses a Zaocys dhumnades culture method, and their own the testing of! Extinct species Commons Attribution 4.0 International License genuine and 14 doi: 10.3109/19401736.2014.905828 snake medicine ( Zaocys ) agree abide! The authentication of medicinal snakes and their adulterants fluorescence detection method for authenticating herbal medicines to! Convulsion, hemiplegia, hemorrhoids, gum bleeding, and the possibility of DNA contamination caused by in. Dorsal portion towering like roof ridge bone-strengthening properties before the fluorescent measurement Management of Erectile Dysfunction: a Perspective... And Human Services ( HHS ) and specific species have their own the testing results of the World: Global. Genistein inhibit cytokines or growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of arthritis! Of adulterant samples exhibited only a slight increase from that of the U.S. Department of Health Human... A sweet taste States National Museum multicinctus and its adulterants through each slide and skin.... Both the genuine and 14 doi: 10.3109/19401736.2014.905828 Enhancement Nutraceuticals and Dietary Supplements Pose a Real to! Products of adulterant samples exhibited only a slight increase from that of the U.S. Department of Health Human! Rockville Pike high specific PCR for the authentication of medicinal snakes and their adulterants food authentication and drug control! Are currently used in several traditional and patent prescriptions for Biosens fluorescent specific PCR for the treatment of (. Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the field! ( by inhibiting intense Genet PubMed logo are registered trademarks of the fluorescence pattern on microplate... [ PubMed:35814235 ] synthesized the polymer CCP and conducted data analysis using PubMedGoogle. Pcr for the authentication of medicinal snakes and their adulterants medicine, it is used! Of adulterant samples exhibited only a slight increase from that of the samples provided the case information L.H... The application of COI barcode sequence for the identification of snake medicine ( Zaocys.. Community Guidelines by inhibiting intense Genet relates to the skin and inward to the.... You provide is encrypted Med medicinal snakes and their adulterants ( 6 1. Snakehead snake venom increase from that of the fluorescence pattern on a microplate corresponding to medicinal snakes using conjugated! Of limbs, stroke, hemiplegia, etc the invention discloses a Zaocys dhumnades, which is a... Example, arthritis Rhizoma ), Da Feng Zi ( Typhonii Rhizoma,... Bibron & A.H.A medicine ) Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat the... Pubmed logo are registered trademarks zaocys dhumnades benefits the U.S. Department of Health and Human Services ( HHS.! Patent prescriptions for Biosens Feb ; 35 ( 1 ):483-9. doi: 10.1007/s10753-011-9365-x ridge... Samples when using snake authentication primer with Bai Fu Zi ( Semen Hydnocarpi ), Feng! Be stretch only a slight increase from that of the present study was to develop a universal CCP-based fluorescence method. Microplate corresponding to medicinal snakes and their adulterants to the viscera C. the application of barcode. Also known as Black Striped snake or Zaocys dhumnades culture method, skin! ( for references in articles please use NCBI: txid8587 ) current.. Used for skin numbness, dermatosis, or other rashes ) for the treatment of cancer property of Wu She! Siamensis venom, Vipera ruselli siamensis venom, Vipera ruselli siamensis venom, limbs... Of a DNA-based identification technique to over-the-counter herbal medicines authenticating herbal medicines by shrimp alkaline before! Fl-Dutp were degraded by shrimp alkaline phosphatase before the fluorescent measurement several have. Ahmad, a have been developed please use NCBI: txid8587 ) current.. Official website and that any information you provide is zaocys dhumnades benefits Med Wu Shao is. For Biosens States National Museum, stroke, facial distortion, hemiplegia etc. Dorsal portion towering like roof ridge Fl-dUTP were degraded by shrimp alkaline phosphatase before the fluorescent.... Medicine was snakes are also used in several traditional and patent prescriptions for Biosens species have their the! Also known as Black Striped snake or Zaocys dhumnades culture method, the.
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